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Mann D.G.,
McDonald S.M., Bayer M.M., Droop S.J.M., Chepurnov V.A., Loke R.E.,
Ciobanu A. and du Buf J.M.H. (2004) The Sellaphora pupula
species complex (Bacillariophyceae): morphometric analysis,
ultrastructure and mating data provide evidence for five new species. Phycologia 43:
459-482
Morphometric shape analysis and ultrastructural data are provided for
six genodemes of the Sellaphora pupula species complex that
have been studied during the last 20 years from Blackford Pond,
Edinburgh. The demes have previously been shown to be separated by
prezygotic reproductive barriers: cells of different demes do not pair,
though they may show residual interactions when sexualized. A new
morphometric method, contour segment analysis, which was recently
developed for diatoms in relation to automated identification, gives a
clear separation of all six genodemes and indicates no heterogeneity
within each. Legendre shape analysis gives less separation in this
instance. All other available data, including molecular sequence data,
also support separation of the six demes at species level. Five new
species are therefore described: S. auldreekie, S.
blackfordensis, S. capitata, S. lanceolata and S.
obesa. The identity of S. pupula sensu stricto is clarified
through designation of epitypes.
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